PADRONIZAÇÃO DA TÉCNICA RT-qPCR PARA DETECÇÃO DE ARBOVÍRUS EM AMOSTRAS DE TECIDOS PARAFINADOS.

Arboviruses are transmitted by mosquitoes and ticks, causing severe diseases in humans such as dengue, Zika, Chikungunya, and yellow fever. This study aimed to standardize the RT-qPCR technique for detecting these viruses in paraffin-embedded tissue samples. A total of 45 paraffin-embedded samples were used, including arboviruses under study and negative control samples from different periods and tissues, cataloged by the Evandro Chagas Institute. Standardization of the RT-qPCR technique is essential for arbovirus surveillance in the Amazon region and Brazil. The results demonstrated efficiency in detecting and quantifying the viral genome of different arboviruses. The RT-qPCR technique proved to be an important tool for the differential diagnosis in paraffin-embedded tissue samples. This standardization is relevant for monitoring and effectively controlling arbovirus-transmitted diseases, contributing to public health and outbreak prevention. This study highlights the importance of standardizing the RT-qPCR technique for detecting arboviruses in paraffin-embedded tissue samples. The Amazon region and Brazil are prone to outbreaks of these diseases, making these advancements even more significant. Early and accurate detection of arboviruses enables the implementation of more effective control and prevention measures, as well as contributing to the understanding of the epidemiology of these diseases. [ABSTRACT FROM AUTHOR]