Investigation of the Effects of Conditioned Medium Derived from Amniotic Membrane on Proliferation in Cancer and Normal Cell Lines.

Introduction: Human amniotic membrane (hAM), obtained after birth, is used in the treatment of various diseases due to its anti-angiogenic, antimicrobial and anti-proliferative properties. In recent years, researches have been focused on the anti-cancer properties of the amniotic membrane. However, reports on this subject are limited. In this study, we aimed to evaluate the anti-cancer effect of the conditioned medium (CM) derived from hAM on various cancer cell lines in vitro. Methods: After cesarean section, placentas of healthy donor mothers were taken and amniotic membrane was obtained. hAM-CM was obtained by incubating amniotic membrane pieces with DMEM with 10% FBS and 1% PSA for 24 hours. HEK293, HUVEC and BV2 for normal cell groups; HEp2, PANC, MDA, A549 for cancer cell groups were cultured. To evaluate the cell viability of hAM-CM in normal and cancer cell lines, XTT test was performed by treating with 75% hAM-CM for 48 hours and the effects on the viability of cancer and normal cells compared to control cells were evaluated. Results:According to the XTT results, after AM-CM treatment, the viability of normal cells decreased by an average of 13% (p<0.05), while there was also a decrease in the viability of cancer cells (HEp2, PANC, MDA, A549) by 4% (ns), 12% (p<0.01), 35% (ns), 18% (p<0.001), respectively. Conclusion: Our results showed that the CM produced from hAM inhibited the proliferation of some cancer cells, while it would not be effective in others. hAM application for breast and lung cancer may be a new hope. [ABSTRACT FROM AUTHOR]