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Engineering of a phosphotriesterase with improved stability and enhanced activity for detoxification of the pesticide metabolite malaoxon.
- Source :
-
PEDS: Protein Engineering, Design & Selection . 2023, Vol. 36, p1-9. 9p. - Publication Year :
- 2023
-
Abstract
- Organophosphorus (OP) pesticides are still widely applied but pose a severe toxicological threat if misused. For in vivo detoxification, the application of hydrolytic enzymes potentially offers a promising treatment. A well-studied example is the phosphotriesterase of Brevundimonas diminuta (BdPTE). Whereas wild-type BdPTE can hydrolyse pesticides like paraoxon, chlorpyrifos-oxon and mevinphos with high catalytic efficiencies, kcat/KM >2 × 107 M−1 min−1, degradation of malaoxon is unsatisfactory (kcat/KM ≈ 1 × 104 M−1 min−1). Here, we report the rational engineering of BdPTE mutants with improved properties and their efficient production in Escherichia coli. As result, the mutant BdPTE(VRNVVLARY) exhibits 37-fold faster malaoxon hydrolysis (kcat/KM = 4.6 × 105 M−1 min−1), together with enhanced expression yield, improved thermal stability and reduced susceptibility to oxidation. Therefore, this BdPTE mutant constitutes a powerful candidate to develop a biocatalytic antidote for the detoxification of this common pesticide metabolite as well as related OP compounds. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17410126
- Volume :
- 36
- Database :
- Academic Search Index
- Journal :
- PEDS: Protein Engineering, Design & Selection
- Publication Type :
- Academic Journal
- Accession number :
- 174979507
- Full Text :
- https://doi.org/10.1093/protein/gzad020