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Computational Design, Combinatorial Screening and Experimental Analysis of Lung Cancer EGFRVIII-Binding Peptides.
- Source :
-
Journal of Computational Biophysics & Chemistry . Mar2024, Vol. 23 Issue 2, p175-188. 14p. - Publication Year :
- 2024
-
Abstract
- Human epidermal growth factor receptor mutation variant III ( EGFR VIII) is a cancer-specific cell surface oncogenic marker and has been observed to be widely involved in the formation, progression and metastasis of lung cancer and some other tumors. Previously, a massive quantity of EGFR VIII -binding peptides were enriched via random phage display (RPD) targeted against the protein. In this study, we reported rational discovery of 12-mer peptides with high affinity to EGFR VIII and strong selectivity for EGFR VIII over wild-type EGFR ( EGFR WT). A combinatorial peptide library was designed to target EGFR VIII based on over ten thousands of known EGFR VIII binders enriched from RPD analysis, and a virtual high-throughput screening protocol was then systematically performed against the library to derive those potential candidates, which were further examined rigorously at structural and energetic levels to identify few promising hits. Anisotropy binding assays were carried out to substantiate the computational findings. Consequently, eight 12-mer peptides were designed as effective binders that can target the EGFR VIII extracellular subdomain 3 (SD3). In particular, two potent peptides (T1: FLHRYEIVTSYF and T3: FLQKYEWNTSYW) were found to have a high affinity to EGFR VIII and a good selectivity for EGFR VIII over EGFR WT . Structural analysis revealed that the peptide-binding site can be divided into hydrophobic, polar and mixed regions, which correspond to the nonpolar N -terminal section, polar/charged middle section and hybrid C-terminal section of the peptide. The peptide selectivity originated from the middle section, which can form a different hydrogen bond network between the two proteins upon the mutating perturbation, whereas the N- and C-terminal sections are primarily responsible for the peptide stability but not specificity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *EPIDERMAL growth factor receptors
*PEPTIDES
*LUNG cancer
*MEDICAL screening
Subjects
Details
- Language :
- English
- ISSN :
- 27374165
- Volume :
- 23
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Computational Biophysics & Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 174880981
- Full Text :
- https://doi.org/10.1142/S2737416523500576