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The Role of Mesenchymal Stem Cells and Imatinib in the Process of Liver Fibrosis Healing Through CCL2-CCR2 and CX3CL1-CX3CR1 Axes.
- Source :
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Reports of Biochemistry & Molecular Biology . Jul2023, Vol. 12 Issue 2, p350-358. 9p. - Publication Year :
- 2023
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Abstract
- Background: Persistent liver damage contributes to the development of liver fibrosis, marked by an accumulation of extracellular matrix. Macrophages play a pivotal role in this process, with the CCL2-CCR2 and CX3CR1-CX3CL1 axes serving as key regulators of macrophage recruitment, liver infiltration, and differentiation. In this study, utilizing a rat model of carbon tetrachloride (CCL4)-induced liver fibrosis, we aimed to investigate the impact of imatinib and bone marrow-derived mesenchymal stem cells (BM-MSCs) on the expression of these axis. Methods: Sixteen Sprague-Dawley rats were divided into four groups: healthy, liver fibrosis, imatinibrecipient, and BM-MSC-recipient. Treatment effects were evaluated using histopathology and Sirus-red staining. Quantitative real-time PCR was employed to analyze changes in the expression of the genes CCL2, CCR2, CX3CL1, and CX3CR1. Results: Histopathological assessments revealed the efficacy of imatinib and BM-MSCs in mitigating liver fibrosis. Our findings demonstrated a significant reduction in CCL2 and CCR2 expression in both imatinib and BM-MSCs treatment groups compared to the liver fibrosis group. Conversely, the gene expression of CX3CL1 and CX3CR1 increased in both therapeutic groups compared to the liver fibrosis groups. Conclusion: Conclusion: The notable decrease in CCL2-CCR2 genes in both therapeutic groups suggests that BM-MSCs and imatinib may contribute to a decline in inflammatory macrophages within the liver. The lower CCL2-CCR2 expression in imatinib-recipient rats indicates better efficacy in modulating the recruitment of inflammatory macrophages. The elevated expression of CX3CL1 in BM-MSC-recipient rats suggests a greater impact on the polarization of LY6Chigh (inflammatory) to LY6Clow (anti-inflammatory) macrophages, warranting further investigation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEPATIC fibrosis
*MESENCHYMAL stem cells
*HEALING
*IMATINIB
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 23223480
- Volume :
- 12
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Reports of Biochemistry & Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 174855324
- Full Text :
- https://doi.org/10.61186/rbmb.12.2.350