生物菌剂与土壤熏蒸互作对三七土壤微生物群落结构的影响.

[Objective] The present paper aimed to investigate the effect of biocontrol agents with dazomet on soil microbial community structure and basic physicochemical properties in order to pave a way for good management in Panax notoginseng cultivation. [Method] A randomized group experiment was designed with edaphic condition (fumigation) and treatment (biocontrol agents) as the two treatment factors. The basic physicochemical properties of the soil were detected and the effect of different treatment factors on the structure of the soil microbial community was analyzed using high throughput sequencing techniques to determine the association between the main physicochemical properties of the soil and the bacterial community. [Result] Soil fumigation increased the abundance of the Proteobacteria, Actinobacteriota and Bacteroidota in the bacterial community, at the same time reduced the abundance of the Gemmatimonadota and Acidobacteriota. The Ascomycota and Basidiomycota were the dominant fungal phylum with the highest abundance in all treatment groups. The soil pH after fumigation significantly decreased compared to that of non-fumigated soil. The bacterial alpha diversity index increased more significantly in the non-fumigated soil added biocontrol agents group, whereas the addition of biofungicide after fumigation reduced fungal alpha diversity. The pH and available nitrogen levels were significantly lower in fumigated sites than in non-fumigated sites under the same treatments, while the addition of biofumigants reduced soil available phosphorus and potassium under the same soil conditions. The pH and available nitrogen levels were significantly lower in fumigated sites than in non-fumigated sites under the same treatments, while the addition of biofumigants reduced soil available phosphorus and potassium under the same soil conditions. Available nitrogen and pH contributed to the strongest correlation with microbial community structure. The addition of biocontrol agents effectively reduced the abundance of Fusarium, Cylindrocarpon, Altemaria and Plectosphaerella and increased the abundance of beneficial genera such as Pseudomonas, Lysobacter and Burkholderia. The addition of biocontrol agents under the same soil conditions significantly reduced the abundance of Moritierella, Fuarium, Aspergillus, Penicillium, Cladosporium which were dominant genera in the fumigated sites. [Conclusion] Non-fumigated land applying biocontrol agents could improve soil acidification during P. notoginseng cultivation. The effect of the biocontrol agents on the alteration of microbial diversity was inconsistent under different soil conditions. Overall, the application of biocontrol agents could improve the microbial community structure of the soil in P. notoginseng cultivation sites and provide an effective method for the prevention and control of P. notoginseng root rot. [ABSTRACT FROM AUTHOR]