葛根种质资源遗传多样性分析及分子身份证构建.

【Objective】A genetic diversity analysis of 21 Pueraria DC. germplasm resources was performed, and the molecular ID was constructed to provide a reference for Pueraria DC. germplasm resources identification.【Method】In this study, twenty-one Pueraria DC. germplasm from different regions were collected as experimental materials. Genetic diversity of Pueraria DC. germplasm was studied by coefficient of variation, Shannon-Weaver diversity index, cluster analysis and ISSR primer polymorphism, and molecular identity card was constructed.【Result】The genetic characters of 21 Pueraria DC. germplasm resources showed rich diversity. Its Shannon-Weaver diversity index ranged from 0.381 to 1.844, with an average of 1.166, and Shannon-Weaver diversity index of main stem diameter was the highest (1.844).The variation coefficient of Pueraria DC. germplasm genetic traits ranged from 16.330% to 55.714%, with an average of 35.157%. Among them, the variation coefficient of mature leaf pattern was the largest (55.714%), and the variation coefficient of other genetic traits was greater than 15.000%.Ten primers with clear bands and good polymorphism were selected from 48 ISSR primers. The polymorphism rate was 40.00%～100%. Polymorphism information content (PIC) value, number of alleles (Na), effective alleles (Ne), Nei’s gene diversity (H’) and Shannon’s information index (I) were 0.7043～0.8468, 1.4000～2.0000, 1.1135～1.4741, 0.0776～0.2999, 0.1276～0.4720, their average values were 0.7800, 1.7830, 1.3514, 0.2160, 0.3353 respectively. These results indicated that the 10 ISSR primers had high polymorphism detection efficiency and were suitable for genetic diversity analysis of Pueraria DC. germplasm resources. The results of cluster analysis could divide the germplasms into 3 groups, and some germplasms had some correlation with geographical origin, and there was mutual introduction or exchange of germplasms from different regions. According to the sequence of PIC values in ISSR markers, P6, P17 and P42 were selected as the core primers, which were suitable for the construction of DNA identity cards of Pueraria DC. germplasm resources.【Conclusion】The 21 germplasm resources of Pueraria DC. have rich genetic diversity and high heterozygosity, which can be used as basic materials for breeding and breeding of Pueraria DC. in future. The selected P6, P17 and P42 can be used as core primers to distinguish 21 Pueraria DC. germplasm resources and construct molecular identity cards. [ABSTRACT FROM AUTHOR]