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Time-resolved fluorescence microspheres-antibody-penicillin-binding protein assisted construction of immunochromatographic assay for sensitive detection of 22 β-lactams in milk.

Authors :
Sun, Yawei
Deng, Xiangjie
Luo, Changwei
Ma, Wentao
Liu, Wanjing
Wang, Jinkui
Li, Qingyue
Bu, Tong
Zhang, Xiya
Source :
Microchimica Acta. Jan2024, Vol. 191 Issue 1, p1-11. 11p.
Publication Year :
2024

Abstract

A sensitive immunochromatographic assay (ICA) using time-resolved fluorescence microspheres (TRFMs) coupled with an indirect-labeling mode was developed for simultaneously determining 22 kinds of β-lactams in milk samples. The TRFMs labeled anti-receptor monoclonal antibodies (mAbs) conjugated to penicillin-binding proteins (PBPs) as ternary TRFMs-mAb-PBPs (TMP) nanoscaffolds provide excellent solubility, brightness, and stability. Thanks to the fact that they not only fully expose the binding sites of PBPs, thereby enhancing the biological affinity of PBPs towards the target, but also generated superb fluorescence signals, the versatile TMP manifested unique possibilities as efficient probes for ICA with remarkable enhancement in sensitivity in β-lactams screening. The results showed that the standard curves of the 22 varying β-lactams displayed linearity in their respective concentration ranges (R2 > 0.98), with the cutoff values of 1–100 ng/mL. The constructed TMP-ICA was successfully applied to the analysis of real milk, with consistent results compared with liquid chromatography-tandem mass spectrometry (LC-MS), providing an effective method for sensing β-lactams in food matrices. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00263672
Volume :
191
Issue :
1
Database :
Academic Search Index
Journal :
Microchimica Acta
Publication Type :
Academic Journal
Accession number :
174799152
Full Text :
https://doi.org/10.1007/s00604-023-06106-1