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Cryopreservation of winter-dormant mulberry buds using two-step freezing.
- Source :
-
International Journal of Industrial Entomology . Dec2023, Vol. 47 Issue 2, p126-133. 8p. - Publication Year :
- 2023
-
Abstract
- Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the ‘‘Daeshim’’ variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33–43% in a low-temperature forced-air chamber at −5 °C, then slowly frozen from −5 °C to −20 °C, and preserved in liquid nitrogen (−196 °C). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the ‘‘Daeshim’’ variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MULBERRY
*GERMPLASM
*FREEZING
*WHITE mulberry
*BUDS
*NATURAL disasters
*SOIL freezing
Subjects
Details
- Language :
- English
- ISSN :
- 15983579
- Volume :
- 47
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- International Journal of Industrial Entomology
- Publication Type :
- Academic Journal
- Accession number :
- 174771466
- Full Text :
- https://doi.org/10.7852/ijie.2023.47.2.126