Screening of betel vine inter-specific hybrids for resistance to Phytophthora nicotianae.

An experiment was conducted to standardize the isolation and screening protocol Phytophthora nicotianae on betel vine to identify the resistance source. The artificial inoculation was carried out by detached leaf assay (pin prick method) and drenching with spore suspension for the root. The result showed that carrot agar was suitable for active culture subsistence, and the pin prick method was the best method for inoculation and screening. Among the inter-specific hybrids used in the present investigation, IIHRPBIH9 recorded significant resistance to Phytophthora foot rot compared to the susceptible check IIHRBV170. Thus, screening of inter-specific hybrids for this disease through artificial inoculation helps to identify resistance sources for the breeding programme, thereby meeting the demand for superior quality, disease-free planting materials and effective disease management. This is the first report for identifying the resistance source in betel vine against P. nicotianae. [ABSTRACT FROM AUTHOR]