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Down-regulated Wnt7a and GPR124 in early-onset preeclampsia placentas reduce invasion and migration of trophoblast cells.
- Source :
-
Journal of Perinatal Medicine . Jan2024, Vol. 52 Issue 1, p41-49. 9p. - Publication Year :
- 2024
-
Abstract
- Preeclampsia (PE) is a disease specific to pregnancy that causes 9–10 % of maternal deaths. Early-onset PE (<34 weeks' gestation) is the most dangerous category of PE. Wnt7a and GPR124 (G protein-coupled receptor 124) are widely expressed in the human reproductive process. Especially during embryogenesis and tumorigenesis, Wnt7a plays a crucial role. However, few studies have examined the association between Wnt7a-GPR124 and early-onset PE. The aim of this study was to examine the significance of Wnt7a and GPR124 in early-onset PE as well as Wnt7a's role in trophoblast cells. Immunohistochemistry (IHC), real-time PCR, and western blotting (WB) were used to investigate Wnt7a and GPR124 expression in normal and early-onset PE placentas. Additionally, FACS, Transwell, and CCK-8 assays were used to diagnose Wnt7a involvement in migration, invasion, and proliferation. In the early-onset PE group, Wnt7a and GPR124 expression was significantly lower than in the normal group, especially in the area of syncytiotrophoblasts (STBs) and extravillous trophoblasts (EVTs). A negative correlation was found between Wnt7a RNA and GPR124 expression (r=−0.42, p<0.01). However, the Wnt7a RNA expression level was positive correlated with PE severity. In further cellular functional experiments, knockdown of Wnt7a inhibits HTR8/SVeno cells invasion and migration but has little effect on proliferation and apoptosis. Through the Wnt pathway, Wnt7a regulates trophoblast cell invasion and migration, and may contribute to early-onset preeclampsia pathogenesis. A molecular level study of Wnt7a will be needed to find downstream proteins and mechanisms of interaction. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IMMUNOCHEMISTRY
*RESEARCH
*TROPHOBLAST
*WESTERN immunoblotting
*WNT proteins
*RNA
*APOPTOSIS
*CELL motility
*PREECLAMPSIA
*GENE expression
*COMPARATIVE studies
*PLACENTA
*GENES
*CELL proliferation
*DESCRIPTIVE statistics
*RESEARCH funding
*POLYMERASE chain reaction
*STATISTICAL correlation
*CHOLECYSTOKININ
*CHEMICAL inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 03005577
- Volume :
- 52
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Perinatal Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 174762207
- Full Text :
- https://doi.org/10.1515/jpm-2022-0565