Steroidal saponins from the roots of Paris verticillata and their anti-proliferative and anti-inflammatory activities.

Thirteen previously undescribed steroidal saponins, named parisverticilloside A-M (1 – 13) and twenty known steroidal saponins (14 – 33) were isolated from ethanol extract of the roots of Paris verticillata. Their structures were identified by a series of spectroscopic methods, including 1D and 2D NMR, HR-ESI-MS, optical rotatory dispersion and chemical processes. The anti-proliferative activities of all compounds against LN229, HepG2, MDA-MB-231 and 4T1 cell lines were evaluated using the CCK8 assay with cisplatin or capecitabine as the positive control. The anti-inflammatory activities of all compounds were measured by inhibition of LPS-induced NO release from BV2 cell lines, with dexamethasone as the positive control. [Display omitted] • Thirteen previously undescribed steroidal saponins were isolated from the roots of Paris verticillata. • The structures were identified by 1D and 2D NMR, HRESIMS, IR, and physical properties. • Steroidal saponins have anti-proliferative and the anti-inflammatory activities. [ABSTRACT FROM AUTHOR]