绞股蓝皂苷对C2C12细胞成肌分化的影响.

Objective It was found that gypenoside not only have anti-inflammatory and anti-aging effects, but also can promote the proliferation and differentiation of osteoblasts and protect their oxidative stress damage. This study aims to investigate the effect of gypenoside on myoblast differentiation of C2C12 cells. Methods C2C12 cells were cultured in complete medium. After cell proliferation and adherent to the wall, differentiation medium containing different concentrations of gypenoside was used to induce cell differentiation, and three repores were set for each concentration. On the fifth day, observed the morphology of cell differentiation and calculated the activity of cell differentiation. Western Blot was used to detect the expression of myoblast markers MYF5 and MyoD1, immunofluorescence staining was used to detect the expression of MyoD1, and Image J software was used to calculate the fusion index of myotube. Results On the fifth day of induction of C2C12 cell differentiation, the cells could differentiate into typical myotube morphology. MYF5 and MyoD1 were significantly expressed under the intervention of 10 mg/L and 5 mg/L gypenoside concentrations, and the fusion index of myotube was also higher with statistically significant differences compared with the control group(P＜0.05). Conclusion Gypenoside can promote myoblast differentiation of C2C12 cells, and the concentration of 10 mg/L and 5 mg/L is better. [ABSTRACT FROM AUTHOR]