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Estudio de la variación genética de la ruta isomerasa del D-galacturonato y análisis estructural de la enzima UxaA (D-altronato deshidratasa) para la transformación de residuos agroindustriales ricos en pectinas a etanol.
- Source :
-
Producción Más Limpia . jul-dic2023, Vol. 18 Issue 2, p78-102. 25p. - Publication Year :
- 2023
-
Abstract
- can cause contamination in body of water or leachate when disposed of in landfills. These residues, such as the shells of some fruits, are very rich in pectin, which cannot be easily fermented by yeasts such as Saccharomyces cerevisiae. Therefore, a search is made for bacteria and fungi that possess this metabolic machinery, in a database built from their nucleotide sequences in a Megablastn, with the DNAsp, Mega, and Jalview software. The isomerase pathway of 4 microorganisms such as Escherichia coli, Lactococcus lactis, Erwinia carotova, Dickeya dadantii, was selected in order to understand the genetic variability. It was obtained that UxaA presented more significant variability in the isomerase pathway. Each UxaA enzyme was modeled by structure predictors. The global protein alignment relationship was found respect to the phylogenetic distance. The isomerase routes of two saprophytic microorganisms Erwinia carotova and Dickeya dadantii are proposed as possible solutions to transfect Saccharomyces cerevisiae as a fermentative alternative to these agro-industrial residues. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BODIES of water
*LACTOCOCCUS lactis
*ISOMERASES
*WATER pollution
*GENETIC variation
Subjects
Details
- Language :
- Spanish
- ISSN :
- 19090455
- Volume :
- 18
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Producción Más Limpia
- Publication Type :
- Academic Journal
- Accession number :
- 174566407
- Full Text :
- https://doi.org/10.22507/pml.v18n2a5