Redox differences between rat neonatal and adult cardiomyocytes under hypoxia.

It is generally accepted that oxidative stress plays a key role in the development of ischemia-reperfusion injury in ischemic heart disease. However, the mechanisms how reactive oxygen species trigger cellular damage are not fully understood. Our study investigates redox state and highly reactive substances within neonatal and adult cardiomyocytes under hypoxia conditions. We have found that hypoxia induced an increase in H 2 O 2 production in adult cardiomyocytes, while neonatal cardiomyocytes experienced a decrease in H 2 O 2 levels. This finding correlates with our observation of the difference between the electron transport chain (ETC) properties and mitochondria amount in adult and neonatal cells. We demonstrated that in adult cardiomyocytes hypoxia caused the significant increase in the ETC loading with electrons compared to normoxia. On the contrary, in neonatal cardiomyocytes ETC loading with electrons was similar under both normoxic and hypoxic conditions that could be due to ETC non-functional state and the absence of the electrons transfer to O 2 under normoxia. In addition to the variations in H 2 O 2 production, we also noted consistent pH dynamics under hypoxic conditions. Notably, the pH levels exhibited a similar decrease in both cell types, thus, acidosis is a more universal cellular response to hypoxia. We also demonstrated that the amount of mitochondria and the levels of cardiac isoforms of troponin I, troponin T, myoglobin and GAPDH were significantly higher in adult cardiomyocytes compared to neonatal ones. Remarkably, we found out that under hypoxia, the levels of cardiac isoforms of troponin T, myoglobin, and GAPDH were elevated in adult cardiomyocytes, while their level in neonatal cells remained unchanged. Obtained data contribute to the understanding of the mechanisms of neonatal cardiomyocytes' resistance to hypoxia and the ability to maintain the metabolic homeostasis in contrast to adult ones. [Display omitted] • Hypoxia induces an increase in H 2 O 2 in adult cardiomyocytes, while neonatal cardiomyocytes show decreased basal H 2 O 2 level. • Hypoxia induces equally strong development of acidosis in adult and neonatal cardiomyocytes. • Adult and neonatal cardiomyocytes demonstrate differences between the electron transport chain (ETC) properties and mitochondria amount. • Hypoxia increases cardiac isoforms of troponin T, myoglobin and GAPDH in adult cardiomyocytes, but not in neonatal cells. [ABSTRACT FROM AUTHOR]