Hormonal and haematological biomarkers as indicators of stress induced by Diuron herbicide toxicity on Clarias gariepinus (Burchell, 1822) sub-adults.

Diuron is a globally used herbicide for weed control but has anti-androgenic effects on androgens (testosterone and androstenedione), antagonist effects on thyroid hormone signaling, and haematological effects due to their biotransformation in fish. Endocrine-disrupting biomarkers such as thyroid hormones, sex hormones, and haematological indices of Clarias gariepinus sub-adults exposed to sub-lethal diuron concentrations were studied over a 28-day period. C. gariepinus (n = 200) sub-adults were exposed to sub-lethal concentrations (0.00, 0.09, 0.18, 0.26, and 0.35 mg/L) of diuron. Changes in the hormonal and haematological profiles of the exposed fish were concentration and exposure duration-dependent. The thyroxine (T4), tri-iodothyronine (T3), and 17β-estradiol (E2) profiles decreased with an increase in concentration and exposure duration. The haemoglobin, pack cell volume, red blood cell, white blood cell, mean cell volume, and mean corpuscular haemoglobin cell decreased, while the mean corpuscular haemoglobin increased with an increase in concentration and exposure duration. Diuron induced stress and altered the physiological mechanisms of fish, and its application in farmlands should be regulated so as to enable a sustainable aquatic eco-system and fishery resources. [Display omitted] • Diuron was toxic even at very low concentration. • Diuron showed endocrine disrupting potential. • The toxicant altered the hormonal and haemoglobin profile of the exposed fish. • The alterations were concentration and exposure duration dependent. • Results showed the need to regulate the application of pesticides on farmlands. [ABSTRACT FROM AUTHOR]