生物炭不同施用量对烟草根际土壤真菌群落结构的影响.

[Objective] The purpose of the paper is to explore the impact of biochar on the population structure, diversity and ecological function of fungi in long-term rhizosphere soil and provide insights for improving the microecological environment of tobacco rhizosphere soil and biochar application in tobacco planting soils. [ Method] In the study,' Honghuadajinyuan' tobacco was utilized as the test material. Under the premise of reducing the fertilizer amount by 10%, 250 g/plant ( A1 ), 300 g/plant ( A2) and 350 g/plant ( A3) of bio-carbon fertilizer were applied, with conventional fertilizer used as the control. Illumina MiSeq high-throughput sequencing technology was employed to determine the rhizosphere soil microorganisms of tobacco plants subjected to these different treatments. [ Result] The application of biochar fertilizer had a significant impact on soil c, diversity and population structure, with A3 treatment demonstrating the highest fungal diversity and abundance among all soil samples. At the phylum level, there was a negative correlation between the relative abundance of Ascomycota and the amount of biochar fertilizer applied, while the relative abundance of Basidiomycota exhibited a positive correlation with the quantity of biochar fertilizer application, both of which were the primary dominant bacteria fungi in each treatment. At the genus level, the application of biochar fertilizer led to a significant decrease in the relative abundance of Penicillium, Cladophialophora, and Clonostachys, while significantly increased the relative abundance of Fusarium, Saitozyma, Conocybe. Addtionally, using FUN Guild for fungal function prediction, it was observed that the functional types of rhizosphere soil fungi in each sample were primarily saprophytic and saprophytic-symbiotic. Furthermore, the relative abundance of plant pathogen in soil samples with biochar fertilizer was lower compared to the control group. [ Conclusion] The study shows that the A3 treatment improves the fungal community structure in the rhizosphere soil of tobacco plants, which is more conducive to maintaining the balance of soil ecosystem and restoring soil quality. It can provide technical reference for rational application of biochar fertilizer in tobacco planting areas. [ABSTRACT FROM AUTHOR]