Relationship between plasma lipid concentrations and HDL subclasses

Abstract: Background: It is generally accepted that different high-density lipoprotein (HDL) subclasses have distinct but interrelated metabolic functions. HDL is known to directly influence the atherogenic process and changes in HDL subclasses distribution may be related to the incidence and prevalence of atherosclerosis. Methods: Apo-AI contents(mg/l) of plasma HDL subclasses were determined by 2-dimensional gel electrophoresis coupled with immunodetection for apo-AI. Four hundred forty-two Chinese adults subjects aged 33 to 78 years were assigned to different groups according to the third Report of NCEP (ATP III) guidelines. The subjects were first divided into 2 groups, normal and high TG, then further classified by plasma TC, HDL-C and LDL-C concentrations. The subjects were also divided into TC desirable and TC high groups. Results: Apo-A contents of preβ1-HDL were higher while HDL2b were lower in high TG subjects vs. the corresponding normal TG subjects according to plasma TC and LDL-C concentrations. With the increase of plasma TC concentrations, apo-AI contents of preβ1-HDL were significantly higher in high TC subgroup vs. TC desirable subgroup in normal TG subjects. With the decrease of HDL-C concentrations, apo-AI contents of HDL2b tended to decrease in normal TG subjects. And, with the increases of LDL-C concentration, in normal TG subjects, apo-AI contents of preβ1-HDL and HDL3b were significantly higher and those of HDL2b were significantly lower in very high LDL-C subgroup vs. LDL-C optimal subgroup. On the other hand, apo-AI contents of preβ1-HDL and HDL3a were significantly higher, while HDL2a and HDL2b were significantly lower in high TG and very high TG subgroup vs. normal TG subgroup within either TC desirable or TC high subjects. In a multivariate linear regression model, TG and TC concentrations were all associated independently and positively with high preβ1-HDL; however, HDL-C were inversely associated with high preβ1-HDL. And TG and TC concentrations were all associated independently and negetively with low HDL2b, but HDL-C and apo-AI were positively associated with low HDL2b. Conclusions: With the increase of plasma TG, TC, LDL-C or the decrease of plasma HDL-C concentrations, there was a general shift toward smaller-sized HDL, which, in turn, indicates that reverse cholesterol transport might be weakened and HDL maturation might be abnormal. Plasma TG concentration is a more important factor than TC concentration on the changes of HDL subclass distribution. Moreover, when TG is normal and HDL-C decreased, large-size HDL particles tended to decrease. [Copyright &y& Elsevier]