Metabolic engineering approaches for scale-up of fermentative biohydrogen production–A review.

Significant efforts are being made to produce biofuels to replace fossil fuels and address the issues caused by global climate change. Due to its potential better conversion efficiency to useable power, decreased emission of pollutants, and high energy density, H 2 is one of the prospective options that are seen as a desirable future clean energy carrier. Although there are numerous technologies available for producing H 2 , this review concentrates on fermentative H 2 production techniques, their drawbacks, and current developments. While being a promising method, fermentative strategies still have several drawbacks, including low H 2 production yields. Many approaches have been used to address these issues; among them, the field of metabolic pathway engineering has made enormous strides. To improve H 2 generation, this paper reviewed and discussed several metabolic pathways and modified strains. As well as the challenges involved in H 2 scale-up from a laboratory setting to a commercial scale. • Biohydrogen production via fermentative methods is reviewed and elaborated. • Metabolic engineering methods are proposed for scaling up fermentative biohydrogen. • Challenges of fermentative biohydrogen production and potential solutions. • Bionanotechnology and modified strains enhanced biohydrogen production. [ABSTRACT FROM AUTHOR]