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Synthesis and glutathione peroxidase (GPx)-like activity of selenocystine (SeC) bioconjugates of biotin and lipoic acid.
- Source :
-
Amino Acids . Dec2023, Vol. 55 Issue 12, p1981-1989. 9p. - Publication Year :
- 2023
-
Abstract
- The conjugation of active biomolecules provides insight into their bioreactivity, leading to many applications in biotechnology and materials science. Herein, we report L-selenocystine (SeC) bioconjugates of lipoic acid (universal antioxidant) and biotin (Vitamin-H). The SeC-bioconjugates, SeC-Biotin (1) and SeC-Lipoic acid (2) were synthesized using solid phase peptide synthesis (SPPS) method and were characterized by multinuclear 1D (1H, 13C, 77Se) and 2D (1H-1H COSY and 1H-13C TOCSY) NMR spectroscopy, ESI–MS spectrometry, and RP-HPLC. The GPx-like enzyme mimicking activity of the SeC-bioconjugates 1 and 2 has been investigated through the coupled reductase assay method for the catalytic reductions of hydrogen peroxide into water. A significant enhancement in GPx-like enzymatic activity was observed for both novel bioconjugates SeC-Biotin (1) and SeC-Lipoic acid (2) as compared to diphenyl diselenide (Ph2Se2), L-selenocystine (SeC), biotin, lipoic acid, and ebselen. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09394451
- Volume :
- 55
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Amino Acids
- Publication Type :
- Academic Journal
- Accession number :
- 174267383
- Full Text :
- https://doi.org/10.1007/s00726-023-03348-4