基因沉默 SlERF14 促进番茄果实成熟.

Tomato (Solanum lycopersicum), the fruit is rich in vitamins, lycopene and other beneficial nutrients to human body, is one of the important vegetable crops. ERF is a type of plant-specific transcription factor, which is an ethylene response factor and can directly regulate the expression of target genes. However, the function of SlERF14 in tomato remains unclear. In this paper,31 ERF gene families in tomato, Arabidopsis thaliana and sweet potato were analyzed by phylogenetic tree, and five ERF genes from a clade with high homology of SlERF14 were screened and summarized for amino acid sequence alignment. The results showed that the function of these genes may be more conservative, and the gene properties and structure are more similar. The expression of SlERF14 gene in tomato was analyzed, and it was found that SlERF14 gene might play a role in fruit ripening. In addition, by virus-induced gene silencing technique (VIGS), it was found that SlERF14 gene silencing promoted the ripening of tomato fruits, resulting in decreased chlorophyll content and increased carotenoid content. After silencing for 15 days, chlorophyll a content was significantly down-regulated (P<0.01), and carotenoid content was significantly up-regulated (P<0.01). The expression levels of carotenoid synthesis related genes (PSY1, PDS, ZDS), chlorophyll degradation related genes (NYC1, PAO, PPH, SGR1), and ethylene anabolic pathway related genes (ACO1, ACO3, ACS2, RIN) all showed an up-regulated trend (P<0.05). It is concluded that SlERF14 gene plays a negative regulatory role in tomato ripening and senescence. In conclusion, the bioinformatics analysis of SlERF14 gene family and its function were studied in this paper, which is of great significance to further study the function of SlERF14 gene in tomato. [ABSTRACT FROM AUTHOR]