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Clade 2.3.4.4 H5 chimeric cold-adapted attenuated influenza vaccines induced cross-reactive protection in mice and ferrets.

Authors :
Weiyang Sun
Jiaqi Xu
Zhenfei Wang
Dongxu Li
Yue Sun
Menghan Zhu
Xiawei Liu
Yuanguo Li
Fangxu Li
Tiecheng Wang
Na Feng
Zhendong Guo
Xianzhu Xia
Yuwei Gao
Source :
Journal of Virology. Nov2023, Vol. 97 Issue 11, p1-24. 24p.
Publication Year :
2023

Abstract

Clade 2.3.4.4 H5Nx subtype avian influenza viruses (AIVs) have circulated in poultry and wild birds worldwide. Recently, an increasing number of H5Nx human infection cases have been reported occurred. Live attenuated influenza vaccines demonstrate more advantages than other types of vaccines, such as ease of administration, elicitation of systemic immune responses, and the ability to generate breadth protection. However, the attenuated viruses also bear the risk of reassortment with the wild-type influenza A virus. To overcome this reassortment problem, we designed and constructed Clade 2.3.4.4 H5 chimeric cold-adapted attenuated influenza vaccines (CAIVs) by introducing the hemagglutinin ectodomains of H5N6 into a cold-adapted attenuated master donor of an influenza B virus. These H5 CAIVs induce humoral antibody response, mucosal immune response, and cellular immune response in mice models. After two doses were administered in mice, H5 CAIVs provided cross-protection responses with 100% survival against wild-type Clade 2.3.4.4 H5 subtype AIVs. The immunized mice exhibited more significant reductions of lung viral titers or lung pathology than those in the mock group mice. In ferret models, Clade 2.3.4.4b and 2.3.4.4h H5 CAIVs produced a cross-protective efficacy against wild-type Clade 2.3.4.4b and Clade 2.3.4.4h H5 AIVs. The findings of the current study indicate that our H5 CAIVs may have the potential to prevent and control H5Nx influenza viruses in humans. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0022538X
Volume :
97
Issue :
11
Database :
Academic Search Index
Journal :
Journal of Virology
Publication Type :
Academic Journal
Accession number :
174087833
Full Text :
https://doi.org/10.1128/jvi.01101-23