Investigation of wound healing potentials of Lysimachia verticillaris and Lysimachia vulgaris: In vivo and in vitro studies.

In this study, the wound healing potential of L. verticillaris and L. vulgaris were investigated in vivo with the "Circular Excision Wound Model". Changes in wound healing have been observed with biological activity and histopathological studies. RT-PCR studies were performed to determine gene expression. Moreover, enzyme inhibitions, antimicrobial activity and HPLC studies were carried out. When the wound area closures were examined, it was determined that the wound closure effect of the ointments was better than the Madecassol®. It was observed that the species contributed to wound healing by decreasing TNF-α, IL-1β and MMP-9 gene levels and increasing IL-10 levels. The collagenase enzyme inhibition values of the species were found to be close to each other and as effective as catechin. It was determined that the species showed the most pronounced antimicrobial activity against S. aureus. Sinapic acid was detected as the major compound in HPLC studies. [Display omitted] • The wound healing potential of the groups that applied ointment containing 5 % extracts were found to be more effective. • It was observed that antioxidant and anti-inflammatory effects were best in groups containing 5 % ointment. • The extracts were reduced the mRNA expression of TNF-α, IL-1β and MMP-9. • Collagenase enzyme inhibition of the extracts was found to be as effective as catechin. • In HPLC studies, sinapic acid was determined as the main component. [ABSTRACT FROM AUTHOR]