AutoEMage: automatic data transfer, preprocessing, real‐time display and monitoring in cryo‐EM.

Cryo‐electron microscopy (cryo‐EM), especially single‐particle analysis, has become a powerful technique for visualizing the structure of biological macromolecules at high resolution. However, data acquisition in cryo‐EM is time consuming because it requires the collection of thousands of images to achieve a high‐quality reconstruction. Real‐time preprocessing and display of the images can greatly enhance the efficiency and quality of data collection. This study presents AutoEMage, a new open‐source software package that automates data transfer, preprocessing and real‐time display in cryo‐EM experiments. AutoEMage also includes a real‐time data monitoring system that alerts users to issues with their data, allowing them to take corrective actions accordingly. The software is equipped with an easy‐to‐use graphical user interface that provides seamless data screening and real‐time feedback on data quality and microscope status. [ABSTRACT FROM AUTHOR]