Study on the breeding of high-yield lignin enzyme strains based on mutagenesis and genome rearrangement.

The study was to efficiently degrade lignocellulose, ligninase high-producing strains were screened and bred by mutation and genome rearrangement. Phanerochaete chrysosporium was used as an original strain and treated with atmospheric room temperature plasma (ARTP) and diethyl sulphate (DES). Mutant strains with higher enzyme productivity were screened by the combination of aniline blue decolorization circle method and guaiacol color circle method and used as starting strains for genome shuffling. Through two rounds of genome rearrangement and genetic stability test. The results showed that the high-yield lignin enzyme fusion PR2-24 was obtained, and its lignin peroxidase activity, manganese peroxidase activity and laccase activity were 11.34, 88.35 and 10.82 U/mL respectively, which were 181%, 171% and 203% higher than those of the original strain. The results showed that the high-yield lignin enzyme strains screened by the test provided a reference for the research and development of microecological feed additives. [ABSTRACT FROM AUTHOR]