苗药花蝴蝶回调CIA 模型大鼠脂肪细胞变化的研究.

Objective To investigate the retracement on related indexes and mechanism of adipocytes changes in CIA model rats with Miao Polygonum runcinatum B.-H.v.S H. Methods A total of 40 rats were randomly divided into normal group. CIA model group methotrexate group. Polygonum runcinatum B.-H. v. S H. high dose group and low dose group. respectively. With 8 rats in each group. According to the relevant literature and the experience of folk medicine, intragastric administration was given respectively. (normal group: normal saline: methotrexate group: 1. 04mg/kg methotrexate. twice a week: Polygonum runcinatum: B.-H. v. S H. high dose group and low dose group: 7. 6.3.8 mg/kg Polygonum runcinatum B. H. v. S H. liquid per day), and stopped after 35 days. The foot swelling of rats in each group was observed, and blood triglyceride (TG), rheumatoid factor (RF). immunoglobulin A (IgA), IgG, IgM and other indicators were observed. Enzyme-linked immunosorbent assay was used to detect adiponectin (ADPN), interleukin-10 (IL-10) and other cytokines were detected and pathological sections of foot joints were studied. The experimental data were statistically processed by SPSS25, 0 software. Results The joint swelling index score and RF-IgM levels in the CIA model group were significantly higher than those in the other groups. IgG level was slightly lower than the Polygonum runcinatum B.-H. v. S H. low dose group and higher than other groups and the differences were statistically significant (P<0.05). The levels of TG. ADPN and IL-10 were significantly lower than those in the other groups. and the differences were statistically significant (P<0,05). In the CIA model group. there were low-grade adipocytes in the bone marrow cavity of the foot joints of the rats and the white blood cells were diffusely proliferated. Compared with other groups. the differences were statistically significant (P<0.05). Conclusion Miao Polygonum runcinatum B.-H. v. S H. may ensure normal energy metabolism (such as TG. etc.) and immunomodulatory factor secretion (such as ADPN. IL-10, etc.) by reversing the reduction of adipocytes in CIA model rats, promote immunomodulatory effects (such as reversing RF. IgG, IgM, etc.) and alleviate foot joint inflammation [ABSTRACT FROM AUTHOR]