OSBPL3 在代谢相关脂肪性肝病中的作用及机制研究.

Objective: To explore the role of OSBPL3 in mediating metabolism-related fatty liver disease and its possible mechanism. Study on the role and mechanism of OSBPL3 in metabolism related fatty liver disease. Hepatic specific silencing OSBPL3 mouse model and empty vector control mouse model were established. They were fed normal diet and high fat diet for 12 weeks respectively. They were divided into normal control group, OSBPL3 silencing group, obese control group and obese OSBPL3 silencing group. To observe the general situation of mice, the lipid synthesis genes and lipid decomposition gene mRNA were detected by Real time PCR, and the key proteins of Akt/mTOR pathway were detected. Human HepG2 cell lines were treated with OA at different concentrations, and the staining changes of O were observed after treatment with different oleic acid concentrations. OSBPL3 expression levels were detected by resting western blot assay. There were no significant differences in all indexes between normal control group and OSBPL3 silencing group (P>0.05). Compared with the control group, the body mass, visceral fat and visceral fat index of the obese control group and the obese OSBPL3 silent group were higher (P<0.05). Compared with the obese control group, the body mass, visceral fat and visceral fat index in the obese OSBPL3 silencing group were lower (P<0.05). Compared with the control group, the TC, TG, LDL-C and HDL-C of the obese control group and the obese OSBPL3 silent group were higher (P<0.05). Compared with the obese control group, the TC, TG, LDL-C and HDL-C in the obese OSBPL3 silenced group were lower (P<0.05). There were no significant differences in the expression levels of SREBP-1C, FAS and PPARα compared with normal control group and OSBPL3 silencing group (P>0.05). Compared with the control group, the levels of SREBP-1C, FAS and PPARα were higher in the obese control group and the obese OSBPL3 silenced group (P<0.05). Compared with the obese control group, the expression levels of SREBP-1C and FAS in the obese OSBPL3 silenced group were lower, and the expression levels of PPARα were higher (P<0.05). Compared with the control group, the expression levels of Akt and mTOR phosphorylation in the obese control group were higher (P<0.05). Compared with the obese control group, the expression levels of Akt and mTOR phosphorylation in the obese OSBPL3 silencing group were lower (P<0.05). Compared with 0 μmol/L oleic acid, oil red O staining was gradually deepened with the increase of OA concentration. Compared with 0 μmol/L oleic acid and 0 μmol/L oleic acid, oil red O staining was gradually deepened with the increase of OA concentration. Compared with 0 μmol/L oleic acid, OSBPL3 mRNA levels of 50 μmol/L, 100 μmol/L and 400 μmol/L oleic acid were increased successively, with statistical difference (P<0.05). The expression level of OSBPL3 increased with the increase of lipid accumulation. OSBPL3 can regulate the expression of lipid metabolism, and play a biological role by regulating Akt/mTOR signal pathway, which is expected to provide references to study the occurrence, development and treatment of MAFLD. [ABSTRACT FROM AUTHOR]