Preliminary Study on Antioxidant Activity and Hair Tonic Spray Formulation of Cocoa Pod Husk (Theobroma cacao L.) Extract.

Introduction: The global prevalence of alopecia has increased significantly in the last decade. So far, the Food and Drug Administration (FDA) has only approved finasteride and minoxidil as chemical substances to treat hair loss. Unfortunately, both drugs may cause various side effects, such as skin irritation, itching, dermatitis, erythema, allergies, and sexual disorders in men. Cream containing cocoa pod husk extract has been reported as a new preparation for hair growth. This study was conducted to test the antioxidant activity and to optimise the formulation of cocoa pod peel extract as a hair tonic spray to stimulate hair growth. Methods: Cocoa pod husk extract was prepared by maceration method using ethanol 96% as a solvent. Qualitative screening and antioxidant activity assay using the DPPH method were performed. The best hair tonic spray formulation was obtained based on organoleptic, pH, and viscosity tests. A sensitivity test was also carried out to ensure the safety of use. Results: The research showed that cocoa pod shell extract contains several active phytochemical compounds. The antioxidant activities test showed that the product has antioxidant activity with IC50 value of 6,508 ppm and based on sensitivity test the hair tonic spray preparation did not cause skin irritation. Conclusion: This product may be promising for future development, but still requires further pre-clinical and clinical testing to ensure the efficacy and safety. [ABSTRACT FROM AUTHOR]