基于西洋参总皂苷调控 TGF-β1/Smad 信号通路 对压力性尿失禁大鼠模型盆底功能恢复的影响.

Objective To explore the effect of TGF-β1/Smad signaling pathway regulated by total saponins of American ginseng on the functional recovery of pelvic floor in rats with stress urinary incontinence. Methods Sixty-five SD rats of SPF grade were selected, and 45 rats after modeling were randomly divided into model group, low-dose group and high-dose group with 15 rats each, and the remaining 15 rats without any treatment were used as control group. Rats in low-dose group and high-dose group were given total saponins of Panax quinquefolium according to the dosage of 50 mg/kg and 100 mg/kg respectively, while rats in control group and model group were given the same amount of physiological saline (15 mL) . The pathological histology, pelvic floor function related indexes, urodynamics related indexes, bladder internal pressure related indexes, pelvic floor muscle surface electrical signals and the relative expression of TGF-β1/Smad signaling pathway protein were compared in each group. Results Compared with those in the control group, urethra rotation angle, bladder neck motion, residual urine volume, urine output, Smad2 and Smad3 expression were increased in the model group, the low and high dose group；vaginal resting pressure, systolic pressure, LPP, MBC, ALPP, urinary efficiency, Class Ⅰ and Class Ⅱ pelvic floor muscle fiber potential, TGF- β1, and Smad7 level were decreased (P < 0.05) ; Compared with those in the model group, vaginal resting pressure, systolic blood pressure, LPP, MBC, ALPP, urinary efficiency, class Ⅰ and Class Ⅱ pelvic floor muscle fiber potential, TGF- β1, Smad7 expression increased in the low and high dose group；urethral rotation angle, bladder neck motion, residual urine volume, urine output, Smad2 and Smad3 levels were decreased (P <0.05) . Conclusion American ginseng total saponin could effectively improve pelvic floor function in rats with stress urinary incontinence, and the mechanism may be related to TGF- β1/Smad signaling pathway. [ABSTRACT FROM AUTHOR]