母乳喂养的新生儿病理性黄疸患儿 肠道菌群结构变化观察.

Objective To observe the structural changes of intestinal flora in breast-fed newborns with pathological jaundice and to provide the theoretical basis for the adjuvant treatment of neonatal pathological jaundice. Methods Thirty breastfeeding infants with pathological jaundice were selected as the observation group and 30 healthy newborns were selected as the control group. The fecal samples from two groups were collected. High-throughput 16S rRNA gene sequencing technology was used to detect RNA of fecal samples in the two groups, β diversity and operational taxonomic units (OTU) were used to analyze the diversity of intestinal flora, and LEfSe was used to screen the dominant intestinal flora (absolute value of LDA score>2), and PICRUSt analysis tool was used to analyze the biological function of intestinal flora. Results Compared with the control group, the number of bacteria OUT in the phylum, class, order, family, genus, species levels was small (all P<0. 05). Compared with the control group, Bacillus abundance was low at the order level, Citrobacter was high, Clostridium pratense was low at the genus level, and Bacaeroides fragilis was low at the species level (all P<0. 05). The dominant intestinal flora of the observation group was Clostridium butyricum, sphingoliomonas and sphingomonas, while the influential intestinal flora of the control group were Bacteroides fragilis and Tricilis. Compared with the control group, the intestinal flora abundance involved in mineral nutrient absorption, spore formation, D-arginine and D-ornithine metabolism, and polyketose unit biosynthesis functional pathways were lower in the observation group (all P<0. 05). The abundances of Clostridium pratense and Bacteroides fragilis were negatively correlated with serum total bilirubin and indirect bilirubin levels in children with neonatal pathological jaundice (all P<0. 05). Conclusion There is structural imbalance and functional differences of intestinal flora in breast-fed newborns with pathological jaundice, and the intestinal flora of children are mainly Clostridium pratense and Bacteroides fragilis. [ABSTRACT FROM AUTHOR]