Metabolic diversity shapes vegetation-enhanced methane oxidation in landfill covers: Multi-omics study of rhizosphere microorganisms.

[Display omitted] • Herbaceous plants were the primary native vegetation in the landfill cover. • Rumex Acetosa increased non-rhizosphere soil CH 4 oxidation capacity nearly 20-fold. • Root exudates induced microbial migration and specific metabolic pathways. • Limonexic acid enhanced methanotrophic bacteria activity and CH 4 degradation. Vegetation root exudates have the ability to shape soil microbial community structures, thereby enhancing CH 4 bio-oxidation capacity in landfill cover systems. In this study, the CH 4 oxidation capacity of indigenous vegetation rhizosphere microorganisms within operational landfill covers in Chongqing, China, was investigated for the first time, with the objective of identifying suitable plant candidates for CH 4 mitigation initiatives within landfill cover systems. Furthermore, a multi-omics methodology was employed to explore microbial community structures and metabolic variances within the rhizospheric environment of diverse vegetation types. The primary aim was to elucidate the fundamental factors contributing to divergent CH 4 oxidation capacities observed in rhizosphere soils. The findings demonstrated that herbaceous vegetation predominated in landfill covers. Notably, Rumex acetosa exhibited the highest CH 4 oxidation capacity in the rhizosphere soil, approximately 20 times greater than that in non-rhizosphere soil. Root exudates played a crucial role in inducing the colonization of CH 4 -oxidizing functional microorganisms in the rhizosphere, subsequently prompting the development of specific metabolic pathways. This process, in turn, enhanced the functional activity of the microorganisms while concurrently bolstering their tolerance to microbial pollutants. Consequently, the addition of substances like Limonexic acid strengthened the CH 4 bio-oxidation process, thereby underscoring the suitability of Rumex acetosa and similar vegetation species as preferred choices for landfill cover vegetation restoration. [ABSTRACT FROM AUTHOR]