儿茶素对阪崎肠杆菌的体外抑制作用.

Enterobacter sakazakii is a food-borne bacterium that causes diseases as diverse as necrotizing enteritis, meningitis and infantile sepsis. Catechin is a kind of natural polyphenol products existing in tea and other natural plants, which has good antibacterial activity. In this study, laser confocal scanning microscopy and field emission scanning electron microscopy observations showed that the cell membrane of E. sakazakii was damaged after the action of catechin, and the cell surface adhered and pitted with holes. Under 1 MIC concentration, E. sakazakii membrane potential increased by 24.79%, intracellular reactive oxygen species increased by 4.72 times, intracellular ATP decreased by 4.93 times, and intracellular pH decreased by 45.63%. These results indicate that catechin can achieve antibacterial effect by disrupting cell membrane integrity and changing cell membrane permeability. This study lays a theoretical foundation for the application of catechin as a natural antibacterial agent. [ABSTRACT FROM AUTHOR]