Back to Search
Start Over
Fabrication of heparinized bi-layered vascular graft with PCL/PU/gelatin co-electrospun and chitosan/silk fibroin/gelatin freeze-dried hydrogel for improved endothelialization and enhanced mechanical properties.
- Source :
-
International Journal of Biological Macromolecules . Dec2023:Part 8, Vol. 253, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Fabricating a biocompatible small-diameter vascular graft (< 6 mm) with mechanical properties similar to the natural vein and adding good anti-thrombogenic, endothelialization, and hyperplasia properties remains a challenge. To this end, we fabricated a heparinized bilayer graft to address this problem. The proposed bilayer sample consisted of a heparinized polycaprolactone (PCL), polyurethane (PU), and gelatin (G) co-electrospun inner layer and chitosan, gelatin, and silk fibroin freeze-dried hydrogel crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) outer layer. The samples exhibited great ultimate stress, Young's module, and suture retention of 4.16 ± 0.25 MPa , 8.24 ± 2.59 MPa and 4.83 ± 0.31 N , respectively. The heparin release assay indicated a sustained release profile of around 70 % after 4 weeks, which can be attributed to the excellent control via emulsion. Furthermore, the heparinized samples demonstrated good anti-thrombogenic properties investigated in the platelet adhesion assay. For the outer layer, the hydrogel crosslinked with non-toxic materials was prepared through the freeze-drying method to achieve high porosity (64.63 %), suitable for smooth muscle cell activity. Moreover, inner and outer layers showed high cell viability toward endothelial (78.96 %) and smooth muscle cells (57.77 %), respectively. Overall, the proposed heparinized graft exhibited excellent potential for vascular graft regeneration. [Display omitted] [ABSTRACT FROM AUTHOR]
- Subjects :
- *VASCULAR grafts
*SILK fibroin
*GELATIN
*POLYCAPROLACTONE
*HYDROGELS
*CHITOSAN
Subjects
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 253
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 173723991
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2023.126807