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Fabrication of heparinized bi-layered vascular graft with PCL/PU/gelatin co-electrospun and chitosan/silk fibroin/gelatin freeze-dried hydrogel for improved endothelialization and enhanced mechanical properties.

Authors :
Almasi-Jaf, Aram
Shamloo, Amir
Shaygani, Hossein
Seifi, Saeed
Source :
International Journal of Biological Macromolecules. Dec2023:Part 8, Vol. 253, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

Fabricating a biocompatible small-diameter vascular graft (< 6 mm) with mechanical properties similar to the natural vein and adding good anti-thrombogenic, endothelialization, and hyperplasia properties remains a challenge. To this end, we fabricated a heparinized bilayer graft to address this problem. The proposed bilayer sample consisted of a heparinized polycaprolactone (PCL), polyurethane (PU), and gelatin (G) co-electrospun inner layer and chitosan, gelatin, and silk fibroin freeze-dried hydrogel crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) outer layer. The samples exhibited great ultimate stress, Young's module, and suture retention of 4.16 ± 0.25 MPa , 8.24 ± 2.59 MPa and 4.83 ± 0.31 N , respectively. The heparin release assay indicated a sustained release profile of around 70 % after 4 weeks, which can be attributed to the excellent control via emulsion. Furthermore, the heparinized samples demonstrated good anti-thrombogenic properties investigated in the platelet adhesion assay. For the outer layer, the hydrogel crosslinked with non-toxic materials was prepared through the freeze-drying method to achieve high porosity (64.63 %), suitable for smooth muscle cell activity. Moreover, inner and outer layers showed high cell viability toward endothelial (78.96 %) and smooth muscle cells (57.77 %), respectively. Overall, the proposed heparinized graft exhibited excellent potential for vascular graft regeneration. [Display omitted] [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01418130
Volume :
253
Database :
Academic Search Index
Journal :
International Journal of Biological Macromolecules
Publication Type :
Academic Journal
Accession number :
173723991
Full Text :
https://doi.org/10.1016/j.ijbiomac.2023.126807