基于极端混合池（BSA）全基因组重测序的羽衣甘蓝 白色叶基因定位.

This study is aimed to screen for key genes and loci associated with white leaf formation in kale (Brassica oleracea L. var. acephala DC) and the genetic patterns of heart leaf color traits. The kale red-leaf parent WR, the white-leaf parent WB and their constructed F2 segregating populations were used as test materials. Twenty red-leaf and white-leaf plants were selected from the F2 population, and two DNA mixing pools were constructed. Whole genome sequencing to offspring mixing pool and parents at 50x and 20x cover depth was conducted respectively, and the association interval of white leaf traits was located and candidate genes were predicted according to the gene annotation information. The results showed that re-sequencing 3 987 718 single nucleic acid polymorphism (SNP) markers were obtained via re-sequencing, 8 significant association intervals on chromosomes 2,3, and 9 were obtained, and 8 candidate genes (Bol030253, Bol029431, Bol012077, Bol007709, Bol030318, Bol030235, Bol030286, and Bol005195) were obtained based on gene annotation and functional analysis. Identifying eight genes as candidate genes for white kale leaves may play an important role in the color formation of kale leaves. [ABSTRACT FROM AUTHOR]