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Pb inhibited C2C12 myoblast differentiation by regulating HDAC2.
- Source :
-
Toxicology . Nov2023, Vol. 499, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Myogenesis is a crucial process governing skeletal muscle development and homeostasis. Lead (Pb) exposure impaired the development and the health of bones, which slows the growth of children. However, it is far from clear what exactly the effects of Pb on skeletal muscle development are. In this study, C2C12 cells are commonly used as an in vitro model of muscle regeneration due to their ability to transition from a proliferative phase into differentiated myofibers. The dose of 1, 5, and 10 μM Pb were adopted to study the toxicity of Pb on C2C12 proliferation and differentiation. First, the effects of Pb on cell viability were detected and the results demonstrated that 5 μM and 10 μM Pb exposure decreased cell viability, while 1 μM Pb exposure has no obvious effects on cell viability. Then, 1–10 μM Pb exposure seriously reduced the C2C12 myoblasts differentiation, with the decrease of myogenic differentiation marker genes expression, including Muscle creatine kinase (MCK), Myosin Heavy Chain 4 (MYH4), Myogenin (MYOG), Myogenic Differentiation (MYOD). What's more, it was found that the epigenetic modifier histone deacetylase-2 (HDAC2) was upregulated after Pb exposure on C2C12 myoblasts. Further studies conclusively showed knockdown of HDAC2 ameliorated Pb-damaged C2C12 myoblasts differentiation, indicating HDAC2 plays a vital role in the Pb-induced C2C12 myoblasts differentiation deficits. In summary, these results demonstrated that Pb exposure inhibited C2C12 myoblasts differentiation by regulating HDAC2. [Display omitted] • Pb exposure retarded myoblast differentiation of C2C12 cells. • Pb exposure upregulated HDAC2 expression during C2C12 myoblast differentiation. • HDAC2 regulated Pb-induced C2C12 myoblast differentiation inhibition. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0300483X
- Volume :
- 499
- Database :
- Academic Search Index
- Journal :
- Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 173698585
- Full Text :
- https://doi.org/10.1016/j.tox.2023.153639