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Anti-inflammatory and urease inhibitory iridoid glycosides from Nyctanthes arbor-tristis Linn.
- Source :
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Journal of Ethnopharmacology . Jan2024:Part 3, Vol. 319, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- Nyctanthes arbor-tristis Linn. has been used by Ayruvedic physicians for the cure of different diseases including ulcers, gastric and inflammatory diseases. To isolate and identify compounds from this source and investigate their therapeutic potential for the treatment of gastric ulcer and related disorders. The ethanol extract of fresh aerial parts of N. arbor-tristis was used in the present studies which was subjected to a bio-assay guided fractionation followed by chromatographic separations. The structures of pure compounds were elucidated using various spectroscopic techniques. The inhibition of urease enzyme was evaluated by weatherburn indophenol method. Molecular docking studies were determined by using Molecular Operating Environment (MOE) version 2020.0901 version. The intracellular ROS production from phagocytes was determined by chemiluminescence assay and NO generation was detected by Griess method. The proinflammatory cytokine TNF-α was quantified by ELISA. Cytotoxic activity was assessed by MTT assay. One previously undescribed iridoid glycoside arborside F (1) and four known iridoid glycosides arborside A (2), arborside C (3), loganin (4) and 7- O-trans -cinnamoyl-6 β -hydroxyloganin (5) were isolated and characterized in the present studies and their urease inhibitory activity was determined. Among these, 2 and 5 showed strong urease inhibition (IC 50 = 12.1 ± 1.74 and 14.1 ± 0.59 μM respectively) (standard acetohydroxamic acid IC 50 = 20.3 ± 0.42 μM), whereas rest of compounds showed moderate to low inhibition. Kinetic studies revealed that compounds 2 and 5 possess competitive type of inhibition. Molecular docking showed polar and non-polar interactions of compounds 2 and 5 with urease enzyme residues. Compounds 2 and 3 showed inhibition of ROS from whole blood (IC 50 = 1.6 ± 0.3 and 2.5 ± 0.09 μg/mL respectively) and PMNs (IC 50 = 1.5 ± 0.03 and 1.4 ± 0.0 μg/mL respectively). Compound 2 significantly inhibited nitric oxide and proinflammatory cytokine TNF-α (IC 50 = 18.2 ± 3.0 and 73.8 ± 6.6 μg/mL respectively). Compounds 1 , 4 and 5 were inactive on ROS. All isolated compounds were non-toxic on normal mouse fibroblasts (NIH-3T3) cells. The ethno pharmacological repute of N. arbor-tristis in treating gastric and anti-inflammatory ailments is supported by present studies which resulted in isolation of a potent urease inhibitory and anti-inflammatory agent arborside A (2) a potential anti-ulcer and anti-inflammatory drug lead. [Display omitted] • Bioguided fractionation, isolation and structure elucidation of urease inhibitory and anti-inflammatory compounds from Nyctanthes arbor-tristis Linn. • Urease inhibitory and anti-inflammatory potential of iridoid glycosides. • Compounds arborside A and arborside C modulate urease enzyme, oxidative species and inflammatory cytokines. • Arborside A showed anti-inflammatory effect by inhibiting ROS, PMNs, NO and TNF-α. • Arborside C showed anti-inflammatory effect by inhibiting ROS, PMNs and NO. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHYTOTHERAPY
*CYTOKINES
*AYURVEDIC medicine
*UREASE
*INFLAMMATION
*ANTI-inflammatory agents
*HETEROCYCLIC compounds
*PLANT anatomy
*ANIMAL experimentation
*GASTROINTESTINAL diseases
*GLYCOSIDES
*TUMOR necrosis factors
*ENZYME-linked immunosorbent assay
*PEPTIC ulcer
*PLANT extracts
*CHROMATOGRAPHIC analysis
*COMPUTER-assisted molecular modeling
*REACTIVE oxygen species
*LUMINESCENCE spectroscopy
*MICE
*CHEMICAL inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 319
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 173696876
- Full Text :
- https://doi.org/10.1016/j.jep.2023.117368