The effect of mesenchymal stem cells on platelet function in rats with experimental lung injury.

Interstitial lung tissue diseases (pulmonary fibrosis) are accompanied by a decrease in thrombopoiesis. Stem cells are capable of differentiating into other cell types, making them a valuable material for veterinary cellular regenerative therapy. The purpose of the research -- to explore changes in platelet counts in laboratory rats with experimental pulmonary fibrosis under the influence of stem cells. The research was performed on female Wistar rats, in which pulmonary fibrosis was modelled using a single transthoracic injection of bleomycin hydrochloride solution. Allogeneic mesenchymal stem cells were used to stimulate recovery processes in pathologically altered lung tissue, which were administered by different routes, and, for comparison, the conventional method of treatment. The presented results of the effect of transplanted allogeneic bone marrow mesenchymal stem cells indicate a significant change in the number and size of platelets in rats with experimental pulmonary fibrosis and an increase in the activity of regenerative processes in damaged tissues. In experimental animals, a significant increase in the number of platelets and their size was found after using allogeneic mesenchymal stem cells compared to similar data in control group animals. In addition, in the blood of animals of the experimental group, which were transplanted with mesenchymal stem cells transthoracically (directly into the lung tissue), there was a higher platelet activity than in animals with intravenous injection of mesenchymal stem cells. Platelet activation indicates an improvement in the regenerative capacity of damaged lung tissue under the influence of mesenchymal stem cells. Thus, transplanted mesenchymal stem cells stimulate platelet activity and regenerative processes in pathologically altered lung tissue in experimental fibrosis, which can be used as one of the effective methods of treating animals with this type of pathology [ABSTRACT FROM AUTHOR]