基于LC-MS/MS多反应监测技术的食管鳞状细胞癌能量代谢物分析.

Objective： To analyze the serum metabolite compositions of the patients with esophageal squamous cell carcinoma (ESCC), to screen out the potential differential metabolites, and to explore the relationship between the occurrence of ESCC and energy metabolism, and to provide the useful energy metabolites for the early diagnosis of ESCC. Methods The serum samples of the ESCC patients and healthy volunteers were collected and divided into ESCC group and control group, and there were 7 cases in each group. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used to detect the serum metabolites of the subjects in two groups；partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models were used to identify the differences in serum metabolites of the subjects between ESCC group and control group, and they were classified according to the chemical classification of serum metabolites; targeted metabolomics analysis on serum energy metabolites was conducted based on the multi-response monitoring (MRM) mode to further identify the significantly differential energy metabolites and their pathways. Results A total of 266 metabolites were identified in the serum samples of the subjects in ESCC group and control group with the positive and negative ion modes, and there were 164 and 102 metabolites identified with the above two modes, respectively. The univariate statistical analysis results showed that there were significant differences in serum metabolites of the subjects between ESCC group and control group. Among the metabolites with chemical classification, lipids and lipid-like molecules accounted for the highest proportion (18.421%) . Three significantly different energy metabolites in ESCC group were found, and the expression levels of L-malic acid and isocitric acid were increased and the expression level of cyclic adenosine monophosphate (cAMP) was decreased. Conclusion There are differences in the expressions of serum energy metabolites between the ESCC patients and healthy controls, and the occurrence of ESCC is related to the energy metabolism. [ABSTRACT FROM AUTHOR]