MiR-491-5p 过表达对人鼻咽癌HONE-1 细胞增殖和迁移的影响.

Objective: To discuss the effect of microRNA-491-5p（miR-491-5p) over-expression on the proliferation and migration of the human nasopharyngeal carcinoma HONE-1 cells, and to provide the evidence for the study of the pathogenesis and targeted therapy of nasopharyngeal carcinoma. Methods: The human nasopharyngeal carcinoma HONE-1 cells were divided into control group (transfected with control plasmid) and miR-491-5p group (transfected with miR-491-5p plasmid). The transfection efficiencies of the HONE-1 cells in two groups were observed under fluorescence microscope; the proliferation activities of the HONE-1 cells in two groups were detected by CCK-8 assay; the number of migration HONE-1 cells in two groups were detected by Transwell chamber assay; the expression levels of Vimentin and E-cadherin mRNA in the HONE-1 cells in two groups were detected by real-time fluorescence quantitative PCR（RTqPCR) method; the expression levels of Vimentin and E-cadherin proteins in the HONE-1 cells in two groups were detected by Western blotting method. Results: Compared with control group, the proliferation activities（t=2. 832, P=0. 0473; t=4. 522, P=0. 0014; t=9. 308, P<0. 01) and number of migration HONE-1 cells (t=9. 639, P<0. 01) in miR-491-5p group at 24, 48, and 72 h after treatment were significantly decreased; compared with control group, the expression levels of Vimentin mRNA (t= 7. 535, P=0. 0017) and protein (t=7. 219, P=0. 0187) in the HONE-1 cells in miR-491-5p group were significantly decreased, and the expression levels of E-cadherin mRNA (t=4. 88, P=0. 0395) and protein (t=5. 754, P=0. 0289) were significantly increased. Conclusion: Over-expression of miR-491-5p can inhibit the proliferation, migration, and epithelial-mesenchymal transition (EMT) the human nasopharyngeal carcinoma cells. [ABSTRACT FROM AUTHOR]