Amorphous amEu-NH2BDC and amTb-NH2BDC as ratio fluorescence probes for smartphone-integrated naked eye detection of bacillus anthracis biomarker.

The abnormal concentration of anthrax spore biomarker 2,6-pyridinedicarboxylic acid (2,6-DPA) will seriously affect public health. Therefore, a sensitive and rapid assay for 2,6-DPA monitoring is of vital importance. In this work, novel nano-sized amorphous Eu-NH 2 BDC (amEu-NH 2 BDC) and amorphous Tb-NH 2 BDC (amTb-NH 2 BDC) metal organic frameworks are prepared by adjusting the ratio of metal and ligand, respectively. Both of them exhibit highly sensitive and selective ratiometric fluorescence detection for 2,6-DPA with wider linear range and lower detection limit in aqueous solutions and human serum. Attributed to the coordination effect of 2,6-DPA in triggering the characteristic fluorescence emissions of Eu3+or Tb3+ by replacing coordinated solvent molecules, as evidenced by ultraviolet–visible spectroscopy, the fluorescence lifetimes analysis, thermal gravimetric analysis, Fourier-transform infrared spectroscopy, density functional theory (DFT) simulations and X-ray photoelectron spectroscopy. In addition, the amEu-NH 2 BDC or amTb-NH 2 BDC loaded paper-based microsensors are constructed for real-time and sensitive detection of 2,6-DPA and coupled with a smartphone-assisted visual portable device. [Display omitted] • Novel nano amEu-NH 2 BDC and amTb-NH 2 BDC were obtained by adjusting the ratio of metal and ligand. • The fluorescence of Eu3+ or Tb3+ in amEu-NH 2 BDC or amTb-NH 2 BDC restored in the presence of 2,6-DPA. • Density functional theory calculation verified the mechanism for detecting 2,6-DPA. • A smartphone-based visual paper sensor was successfully detected 2,6-DPA in human serum. [ABSTRACT FROM AUTHOR]