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Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress.
- Source :
-
Journal of Vascular Research . 2023, Vol. 60 Issue 4, p204-212. 9p. - Publication Year :
- 2023
-
Abstract
- Objectives: This study aimed to determine the function of Cx43 in the endothelial-to-mesenchymal transition (EndMT) process of endothelial cells (ECs) and to explore the potential signaling pathways underlying these functions. Methods: ECs were extracted from rat aorta. ECs were transfected with Cx43 cDNA and Cx43 siRNA and then exposed to 5 or 12 dyne/cm2. Immunofluorescence staining was used to detect the expression of SM22α, Cx43, and acetylated α-tubulin in ECs. Western blotting was used to detect the protein expression of α-SMA, CD31, Cx43, H1-calponin, Ift88, and p-smad3 in ECs. Results: The expression of αSMA, SM22α, and Cx43 was significantly increased, and CD31 was markedly decreased in ECs treated with laminar shear stress at 5 dyn/cm2. The Cx43 cDNA transfection could induce the expression of SM22α or H1-calponin and attenuate CD31 expression in ECs. Also, Cx43 overexpression harms cilia formation in ECs exposed to 5 dyn/cm2, accompanied with the regulated Ift88 and smad signaling. Conclusions: This study found that laminar shear stress at 5 dyn/cm2 would increase the expression of Cx43 to facilitate the EndMT process of ECs, associated with morphological changes in primary cilia and the decreased expression of Ift88 in ECs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10181172
- Volume :
- 60
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Vascular Research
- Publication Type :
- Academic Journal
- Accession number :
- 173420357
- Full Text :
- https://doi.org/10.1159/000533320