Establishment of a dual-signal sandwich sensor for detection of shigella sonnei based on truncated aptamers.

Shigella Sonnei (S. sonnei) is a common foodborne pathogen causing intestinal diseases. In this study, a sandwich-structured colorimetric and surface-enhanced Raman scattering (SERS) dual methods adaptation sensor was prepared for the detection of S. sonnei. Firstly, two aptamers, SS4.1-2 (K d = 43.5 ± 2.78 nM) and SP1-2.2 (K d = 23.3 ± 3.01 nM) with good specificity and affinity were obtained by truncation strategy. Then, Fe 3 O 4 @SiO 2 @Ag nanoparticles were utilized to capture bacteria, while Cu-TCPP (Fe)/AuNPs nanosheets were used as signal probes due to their oxidase activity and SERS signals. The detection limits of the aptasensor were as low as 37 CFU/mL for the colorimetric signal and 7 CFU/mL for SERS signal. Furthermore, the aptasensor was successfully applied to detect S. sonnei in real samples with 94.95–112.92% and 98.40–103.95% recoveries for colorimetric and SERS methods, respectively. A more rapid and accurate method to detect S. sonnei contamination in food was improved. • Two aptamers that specifically bind to S. sonnei were truncated and optimized to avoid the high cost and instability. • The Fe 3 O 4 @SiO 2 @Ag nanoparticles were synthesized and used as capture probes with separation and enrichment properties. • The Cu-TCPP (Fe)/AuNPs nanosheets were successfully synthesized and used as detection probes with peroxidase properties and SERS signals. • Development of an aptamer sandwich dual signal sensor for S. sonnei. [ABSTRACT FROM AUTHOR]