枳壳不同生育期根际土壤细菌群落变化研究.

[Objective] The study aimed to analyze the composition of rhizosphere soil bacterial community in different growth stages of Fructus aurantii and its relationship with soil environmental factors, reveal the key environmental factors that affect the structure of soil bacterial community, and provide scientific guidance for rational cultivation management and soil cultivation of F. aurantii. [Method] The rhizosphere soil samples of F. aurantii at flowering, fruit setting, harvesting and overwintering stage were collected respectively. Then the diversity index and community composition of bacteria was determined by high-throughput sequencing technology. The soil physicochemical properties and enzyme activities were measured by chemical analysis methods. The correlations between bacterial community structure and factors were analyzed by R software. [Result]The soil pH was significantly higher in the harvesting stage than in other stages(P<0.05,the same as below) ; The total nitrogen, available potassium and activity of catalase were significantly higher in fruit setting stage than in flowering and harvesting stage. A total of29 240 bacterial OTUs were obtained by high-throughput sequencing, which belonged to 409 genus, 253 families, 158 orders, 72 classes and 24 phyla. The Chaol index was higher in fruit setting stage than other stages. At phylum level, the dominant was Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria and Gemmatimonadetes, which accounted for 82. 53 % - 88. 49%. At the genus level, the dominant was Sphingomonas,RB41,MNDJ,Nitrospira,Subgroup_lO,Haliangium,Bryobacter and Dongia, which accounted for 13.16% -19. 49%. The abundance of Dongia was higher in fruit setting stage than other stages, while the Subgroup_JO was significantly increased in overwintering stage. The correlation analysis showed that the abundance of Haliangium, Bryobacter, Lysobacter were significantly positively correlated with pH, organic matter and activity of urease, while the Subgroup_JO was significantly negatively correlated with available nitrogen. [Conclusion] The rhizosphere soil nutrients, activities of enzymes and bacteria community composition were dynamically changed at growth stages, and the bacterial community structure was mainly determined by soil pH and available nitrogen in F. aurantii. [ABSTRACT FROM AUTHOR]