血必净通过miR-155/JAK2/STAT1信号通路对肺炎 克雷伯菌所致重症肺炎大鼠肺组织损伤的影响

Objective To investigate the effect of Xuebijing on rats with severe pneumonia （SP） and its possible mechanism. Methods Forty SP rats were randomly divided into model group, miRNA-155 agonist group （miRNA-155 agonist, 80 mg/kg）, Xuebijing low-dose group （Xuebijing injection, 4 mg/kg）, Xuebijing high-dose group （Xuebijing injection, 8 mg/kg）, miR-155 agonist + Xuebijing group （miR-155 agonist 80 mg/kg, Xuebijing injection 8 mg/kg）, with 8 rats in each. Eight healthy rats were included into control group. Control group and model group were injected with the same amount of normal saline through caudal vein. After 7 days of continuous administration, lung coefficient （LI）, interleukin-1β （IL-1β） level, receptor tyrosine kinase 2 （JAK2）, signal transduction and transcriptional activator 1 （STAT1） protein expression were detected in each group. Results Compared with those in the model group, the level of LI and IL-1β, p-JAK2/JAK2 and p-STAT1/STAT1 in lung tissue were increased in miR-155 agonist group （P < 0.05）. The level of LI and IL-1β, P-JAK2/JAK2 and P-Stat1/STAT1 in lung tissue were decreased in low and high dose Xuebijing group and miR-155 agonist + Xuebijing group （P < 0.05）. Compared with those in miR-155 agonist group, the level of LI and IL-1β, P-JAK2/JAK2 and P-Stat1/STAT1 in lung tissue were decreased in Xuebijing low and high dose group, and miR-155 agonist + Xuebijing group （P < 0.05）. Compared with those in Xuebijing low dose group, the level of LI, IL-1β, p-JAK2/JAK2 and p-STAT1/STAT1 in lung tissue were increased in Xuebijing high dose group （P < 0.05）. Compared with those in Xuebijing group, LI and IL-1β level, P-JAK2/JAK2 and P-Stat1/STAT1 in lung tissue were increased in miR-155 agonist + Xuebijing group （P < 0.05）. Conclusion Xuebijing can reduce lung injury in SP rats, possibly by inhibiting the expression of miR-155 and thereby reducing the JAK2/STAT1 signaling pathway. [ABSTRACT FROM AUTHOR]