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Acteoside protects podocyte against apoptosis through regulating AKT/GSK-3β signaling pathway in db/db mice.
- Source :
-
BMC Endocrine Disorders . 10/23/2023, Vol. 23 Issue 1, p1-11. 11p. - Publication Year :
- 2023
-
Abstract
- Background: Podocyte apoptosis is one of the important pathological mechanisms of diabetic kidney disease (DKD). Acteoside (Act), a major active component of Rehmannia glutinosa leaves total glycoside, has a strong renoprotective action. Our study aims to demonstrate Act's renoprotective actions in db/db mice. Methods: We adopted C57BLKS/J db/db mice as DKD animal models. After 8 weeks of Act administration, the 24-hour urine albumin, renal function index, and blood lipid levels were quantified using matching kits. Renal pathology was evaluated by HE and PAS staining. The podocyte damage and apoptosis-related signaling pathway were observed by using immunohistochemistry, western blot, and TUNEL staining. Results: The albuminuria of db/db mice was reduced from 391 ug/24 h to 152 ug/24 h, and renal pathology changes were alleviated after Act administration. The western blot and immunohistochemistry showed that Act treatment upregulated the synaptopodin and podocin expression compared with db/db mice, while the TUNEL staining indicated podocyte apoptosis was inhibited. The B-cell lymphoma-2 (Bcl-2) level was upregulated in the Act group, but cleaved caspase-3 and Bcl-2 associated X protein (Bax) expression declined, while the protein kinase B/glycogen synthase kinase-3β (AKT/GSK-3β) signaling pathway was repressed. Conclusions: By inhibiting the AKT/GSK-3β signaling pathway, Act protected podocytes from apoptosis, decreasing the urine albumin of db/db mice and delaying the course of DKD. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KIDNEY physiology
*PROTEIN kinases
*BIOLOGICAL models
*ALBUMINS
*STAINS & staining (Microscopy)
*ANIMAL experimentation
*IMMUNOHISTOCHEMISTRY
*WESTERN immunoblotting
*APOPTOSIS
*SIGNAL peptides
*DIABETES
*GLYCOSIDES
*CELLULAR signal transduction
*PREVENTIVE health services
*COMPARATIVE studies
*TRANSFERASES
*DESCRIPTIVE statistics
*RESEARCH funding
*EPITHELIAL cells
*DIABETIC nephropathies
*MICE
*LIPIDS
*ALBUMINURIA
Subjects
Details
- Language :
- English
- ISSN :
- 14726823
- Volume :
- 23
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- BMC Endocrine Disorders
- Publication Type :
- Academic Journal
- Accession number :
- 173147347
- Full Text :
- https://doi.org/10.1186/s12902-023-01483-3