Dynamic tandem proximity‐based proteomics—Protein trafficking at the proteome‐scale.

Fluorescein-tagged proteins are first purified using anti-fluorescein-based immunoprecipitation of the lysate followed by streptavidin coated bead-based pull-down of the first eluate resulting in the enrichment of doubly-labeled proteins, corresponding to their presence in both donor and acceptor compartments. Keywords: new methodology; proximity labeling; TransitID EN new methodology proximity labeling TransitID 546 548 3 10/25/23 20231101 NES 231101 TransitID is a new methodology based on proximity labeling allowing for the study of protein trafficking a the proteome scale. These purified proteins can then be analyzed using tandem mass spectrometry. gl First, the proteome in a donor compartment (localization #1, Figure 1) is biotin-labeled with a compartment-resident TurboID-tagged protein. [Extracted from the article]