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Involvement of the TRAP-1 homologue, Dd-TRAP1, in spore differentiation during Dictyostelium development
- Source :
-
Experimental Cell Research . Feb2005, Vol. 303 Issue 2, p425-431. 7p. - Publication Year :
- 2005
-
Abstract
- Abstract: Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a member of the molecular chaperone HSP90 (90-kDa heat shock protein) family. We have previously demonstrated that Dictyostelium discoideum TRAP1 (Dd-TRAP1) synthesized at the vegetative growth phase is retained during the whole course of D. discoideum development, and that at the multicellular slug stage, it is located in prespore-specific vacuoles (PSVs) of prespore cells as well as in the cell membrane and mitochondria. Thereupon, we examined the function of Dd-TRAP1 in prepore and spore differentiation, using Dd-TRAP1-knockdown cells (TRAP1-RNAi cells) produced by the RNA interference method. As was expected, Dd-TRAP1 contained in the PSV was found to be exocytosed during sporulation to constitute the outer-most layer of the spore cell wall. In the TRAP1-RNAi cells, PSV formation and therefore prespore differentiation were significantly impaired, particularly under a heat stress condition. Although the TRAP1-RNAi cells formed apparently normal-shaped spores with a cellulosic wall, the spores were less resistant to heat and detergent treatments, as compared with those of parental MB35 cells derived from Ax-2 cells. These findings strongly suggest that Dd-TRAP1 may be closely involved in late development including spore differentiation, as well as in early development as realized by its induction of prestarvation response. [Copyright &y& Elsevier]
- Subjects :
- *TUMOR necrosis factors
*MOLECULAR chaperones
*HEAT shock proteins
*CELL membranes
Subjects
Details
- Language :
- English
- ISSN :
- 00144827
- Volume :
- 303
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Experimental Cell Research
- Publication Type :
- Academic Journal
- Accession number :
- 17309951
- Full Text :
- https://doi.org/10.1016/j.yexcr.2004.10.010