西咪替丁对坏死性小肠结肠炎小鼠炎症免疫反应、肠道微生物的影响.

Objective: To investigate and analysis the effects of cimetidine on inflammatory immune response and intestinal microorganism in mice with necrotizing enterocolitis (NEC). Methods: 36 cases of mice with necrotizing enterocolitis were randomly divided into three groups: model group, cimetidine group 1, and cimetidine group 2, with 12 mice in each group. The model group, cimetidine group 1, and cimetidine group 2 were orally administrated with 0.1 mL of normal saline, 0.1 mL of gastric cimetidine, and 0.2 mL of gastric cimetidine daily. After continuous application for 14 days, the inflammatory immune response and intestinal microbial changes of the mice were observed. Results: The body weight of cimetidine group 1 and cimetidine group 2 on the 7th and 14th day of treatment were significantly higher than that of the model group(P<0.05), and the body weight of cimetidine group 2 were significantly higher than that of cimetidine group 1(P<0.05). At the 14th day of treatment, the content of IL-2 in intestinal tissue of cimetidine group 1 and cimetidine group 2 were lower than that of model group (P<0.05), and the content of IL-10 were higher than that of model group(P<0.05). There were significant difference compared between cimetidine group 2 and cimetidine group 1 (P<0.05). At the 14th day of treatment, the relative expression levels of Bifidobacterium and Lactobacillus in the feces of cimetidine 1 and 2 groups were significantly higher than those of the model group(P<0.05), while the relative expression levels of Escherichia coli were significantly lower than those of the model group (P<0.05). There were significant difference compared between cimetidine 2 and cimetidine 1 groups (P<0.05). The relative expression levels of CaMKIV and CREM proteins in the intestinal tissue of cimetidine 1 and 2 groups were significantly lower than those of the model group on the 14th day of treatment(P<0.05), and cimetidine 2 group also significantly decreased compared with cimetidine 1 group (P<0.05). Conclusion: The application of cimetidine in mice with necrotizing enterocolitis can regulate the balance of inflammatory immune response, promote the recovery of mice weight to normal, and also reduce the relative expression level of CaMKIV and CREM proteins in intestinal tissue, thus improving the intestinal microbial status of mice, which is dose dependent. [ABSTRACT FROM AUTHOR]