紫花地丁香豆素类提取物 体外对新城疫病毒抑制作用的研究.

The experiment was to study the inhibitory effect of coumarin extract from Viola yedoensis Makino on Newcastle disease virus in vitro. The experiment used Viola yedoensis Makino as the material to prepare the coumarin extract, used high performance liquid chromatography to detect the content of aesculin and aesculetin in the extract, and used CCK-8 method to detect the effect of extract on Newcastle disease virus (NDV) infection of chicken embryo fibroblasts. The results showed that the contents of aesculin and aesculetin in Viola yedoensis Makino were 2.58, 7.66 mg/g, respectively. The median lethal concentration of coumarin extract from Viola yedoensis Makino on DF-1 cells was 185.2 mg/L. Coumarin extract from Viola yedoensis Makino inhibits NDV replication in a dose-dependent manner. When the extract concentration was 100 mg/L, the replication inhibition rate against NDV virus was the highest with the value of 74.31%. In the inhibition of NDV adsorption, when the concentration of lycopene extract was 100 mg/L, the inhibition rate of NDV virus was the highest with the value of 67.4%. The study indicates that the coumarin extract from Viola yedoensis Makino has obvious anti-NDV effect in vitro. [ABSTRACT FROM AUTHOR]