Back to Search
Start Over
Comprehensive analysis of aflatoxin B1 biosynthesis in Aspergillus flavus via transcriptome-wide m6A methylome response to cycloleucine.
- Source :
-
Journal of Hazardous Materials . Jan2024, Vol. 461, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Aspergillus flavus and its toxic aflatoxins secondary metabolites contaminate food and grains, posing a severe threat to human health and leading to liver cancer. Here, we demonstrated that cycloleucine blocked aflatoxin B 1 synthesis by inhibiting N6-methyladenosine (m6A) methylation modification of messenger RNA (mRNA). m6A Methylation Immunoprecipitation Sequencing (m6A MeRIP-Seq)-based comprehensive transcriptome-wide m6A profiling identified 102 differentially expressed genes that underwent m6A modification, of which 22 hypermethylated genes were downregulated and 49 hypomethylated genes were upregulated, suggesting a negative correlation between m6A methylation and gene expression. Notably, cycloleucine inhibited aflatoxin B 1 production via multiple targets. The m6A sites of several key genes involved in the aflatoxin B 1 biosynthesis pathway were significantly enriched in the coding sequence and around the stop codon, resulting in their downregulation. Furthermore, m6A methylation on genes related to the aflatoxin B 1 biosynthesis pathway led to reduced mRNA stability. Cycloleucine inhibition of aflatoxin B 1 production highlights its potential as an agent for removing mycotoxins in environmental pollution. Aflatoxins, highly carcinogenic secondary metabolites produced by Aspergillus flavus , frequently contaminate crops such as peanut, corn, wheat and sesame leading to irreversible loss in the quality and yield of agricultural products and posing serious threats to food safety. Aflatoxins has also been linked to developmental delays and liver cancer in humans. In our study, 'monitoring aflatoxin concentrations and its bioaccumulation in organisms' has been conducted. The results demonstrated that aflatoxin production in A. flavus was completely blocked after cycloleucine treatment. Additionally, we demonstrated that inhibition of aflatoxin was linked to N6-methyladenosine methylation of multiple genes in aflatoxin biosynthesis pathway. [Display omitted] • Aflatoxin B 1 production was completely blocked after cycloleucine treatment. • Cycloleucine significantly inhibited the pathogenicity of A. flavus to infect grains. • Cycloleucine inhibited aflatoxin B 1 production via modulating m6A methylation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03043894
- Volume :
- 461
- Database :
- Academic Search Index
- Journal :
- Journal of Hazardous Materials
- Publication Type :
- Academic Journal
- Accession number :
- 173010053
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2023.132677